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. 2022 Nov 25;13:1008154. doi: 10.3389/fphys.2022.1008154

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Copyright © 2022 Damulewicz, Tyszka and Pyza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lack of light changes neurotransmitters signaling level. qPCR analysis of expression level of genes connected with histamine, octopamine, PDF and sNPF signaling for flies maintained in normal conditions LD12:12 (LD/LD), maintained in DD and developed in darkness (DD/DD) and after eclosion transferred to LD12:12 (DD/LD). Expression checked for: (A) histidine decarboxylase (Hdc), (B) receptor HisCl1, (C) receptor Ort. (D) Tbh and (E) receptor Oamb, (F) Pdf, (G) Pdfr, (H) sNPF and (I) sNPF-R. N = 5. Mean ± SEM.