FIG. 5.
SDS-PAGE and Western blotting of parent strain 35000 and dsrA mutant FX517. (A) Outer membranes were prepared, solubilized at 37 or 100°C, and subjected to SDS-PAGE and Coomassie blue staining. (B) For the Western blot, outer membranes were solubilized at 100°C, transferred to nitrocellulose, and probed with anti-DsrA mouse serum. Bound antibody was detected with alkaline phosphatase-conjugated secondary antibody and BCIP/NBT substrate. The asterisks indicate the positions of the DsrA protein. STD, molecular weight standards.