FIG. 7.

Complementation of dsrA mutants and bactericidal killing. (A) Total cellular proteins from the indicated H. ducreyi strains were subjected to SDS-PAGE (12% polyacrylamide) and Western blotting with anti-DsrA antisera. Bound antibody was detected with horseradish peroxidase-conjugated secondary antibody followed by chemiluminescence. Plasmids: A, pUNCH 1260 (contains the entire dsrA ORF from strain 35000 and its putative native promoter as illustrated in Fig. 2); B, pLSKS (vector without insert). (B) Bactericidal killing of the complemented dsrA mutants. Bactericidal killing was performed as in the experiment in Fig. 1 (50% serum), except that the medium used contained streptomycin. %, percent survival. The data are compiled from separate experiments done on at least three different days.