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. 2000 Mar;68(3):1608–1619. doi: 10.1128/iai.68.3.1608-1619.2000

TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid Relevant genotype or phenotype Source, reference, or locale isolated
E. coli K-12
 DH5αMCR recA gyrB Bethesda Research Laboratories
H. ducreyi
 35000 Wild type, parent Stanley Spinola Indiana Univ.
 FX516 35000 cointegrate; β-galactosidase positive; intermediate in FX517 construction; Cmr This work
 FX517 35000 dsrA Cmr This work
 CIP 542 (CAN) Wild type (avirulent) William Albritton (13)
 CIP A77 Wild type (avirulent) Robert Munson (31)
 CIP 542 (CDC) Wild type (virulent) Stephen Morse (49)
H. ducreyi obtained from Pat Totten
 CIP A75 Wild type (avirulent) Pasteur Institute (31)
 CHIA Wild type VDRL (12)
 V-1157 Wild type Seattle (12)
 M90-02 Wild type Bahamas
 406 Wild type Mississippi
Plasmids
 pCRII PCR cloning vector; Kanr Ampr Invitrogen
 pUNCH 1248 dsrA PCR clone using primers 14 and 16 in pCRII vector This work
 pLS88 Shuttle plasmid; Kanr Strr Sulr (11)
 pUNCH 1254 dsrA subclone; EcoRI fragment of pUNCH 1248 in EcoRI of pLS88 This work
 pUNCH 1255 Mutagenized dsrA; pUNCH 1254 mutagenized with CAT cassette from pNC40; Kanr Cmr This work
 pRSM1791 Mutagenesis plasmid; β-gal+ Ampr (7)
 pUNCH 1256 pUNCH 1255 (SmaI-HincII-Klenow) into the NotI site (Klenow) of pRSM1791 This work
 pUNCH 1260 dsrA PCR clone, using primers 14 and 16 in pLSKS This work
 pNC40 Source of CAT cassette; Ampr Cmr (44)
 pLSKS Cmr (55)
 pBluescript Ampr Stratagene
 pMCL210 Cmr (29)
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