TABLE 1.
Bacterial strains and plasmids used in this study
Strain or plasmid | Relevant genotype or phenotype | Source, reference, or locale isolated |
---|---|---|
E. coli K-12 | ||
DH5αMCR | recA gyrB | Bethesda Research Laboratories |
H. ducreyi | ||
35000 | Wild type, parent | Stanley Spinola Indiana Univ. |
FX516 | 35000 cointegrate; β-galactosidase positive; intermediate in FX517 construction; Cmr | This work |
FX517 | 35000 dsrA Cmr | This work |
CIP 542 (CAN) | Wild type (avirulent) | William Albritton (13) |
CIP A77 | Wild type (avirulent) | Robert Munson (31) |
CIP 542 (CDC) | Wild type (virulent) | Stephen Morse (49) |
H. ducreyi obtained from Pat Totten | ||
CIP A75 | Wild type (avirulent) | Pasteur Institute (31) |
CHIA | Wild type | VDRL (12) |
V-1157 | Wild type | Seattle (12) |
M90-02 | Wild type | Bahamas |
406 | Wild type | Mississippi |
Plasmids | ||
pCRII | PCR cloning vector; Kanr Ampr | Invitrogen |
pUNCH 1248 | dsrA PCR clone using primers 14 and 16 in pCRII vector | This work |
pLS88 | Shuttle plasmid; Kanr Strr Sulr | (11) |
pUNCH 1254 | dsrA subclone; EcoRI fragment of pUNCH 1248 in EcoRI of pLS88 | This work |
pUNCH 1255 | Mutagenized dsrA; pUNCH 1254 mutagenized with CAT cassette from pNC40; Kanr Cmr | This work |
pRSM1791 | Mutagenesis plasmid; β-gal+ Ampr | (7) |
pUNCH 1256 | pUNCH 1255 (SmaI-HincII-Klenow) into the NotI site (Klenow) of pRSM1791 | This work |
pUNCH 1260 | dsrA PCR clone, using primers 14 and 16 in pLSKS | This work |
pNC40 | Source of CAT cassette; Ampr Cmr | (44) |
pLSKS | Cmr | (55) |
pBluescript | Ampr | Stratagene |
pMCL210 | Cmr | (29) |