Fig. 2.

Knockdown of CHCHD4 repressed cell proliferation and accelerated apoptosis in LUAD cells. After si-CHCHD4-1, si-CHCHD4-2 and si-CHCHD4-3 were transfected into A549 cell, CHCHD4 expression levels were evaluated using western blot (A) and qRT-PCR (B); cell viability was examined using CCK-8 assay (C); cell colonies were analysed with colony formation assay (D); proliferation was tested using EdU assay (E); apoptosis was assessed using FITC-Annexin V/PI apoptosis detection kit (F); the levels of apoptosis-related proteins were evaluated using western blot (G). ^*P < 0.05