Fig. 5.

Knockdown of CHCHD4 inhibited the progression of LUAD via suppressing the MYC pathway. A After si-CHCHD4-1, si-CHCHD4-2, and si-CHCHD4-3 were transfected into A549 and H1299 cells, the protein levels of CHCHD4, and MYC were determined using western blot. B–C After pcDNA3.1-MYC vector was transfected into A549 cells, MYC expression was determined using western blot and qRT-PCR. After pcDNA3.1-MYC vector and si-CHCHD4-2 were co-transfected into A549 cells, cell viability was analysed using the CCK-8 assay (D); apoptosis was assessed using FITC-Annexin V/PI apoptosis detection kit (E); migration and invasion were examined by applying the transwell assay (F). ^*P < 0.05, ^#P < 0.05, ^&P < 0.05