TABLE 1.

Primers for RT-PCR analysis of lung adhesion molecule, chemokine, and cytokine expression

Target	Size of product (bp)	Sequence
GAPDH sense	460	5′-TGC-ATC-CTG-CAC-CAC-CAA-CT-3′
GAPDH antisense	460	5′-GCC-CCA-GCA-TCA-AAG-GTG-G-3′
ICAM-1 sense	485	5′-GCG-GCC-TTG-GAG-GTG-GAT-G3′
ICAM-1 antisense	485	5′-GGA-GGC-GGG-GCT-TGT-ACC-3′
VCAM-1 sense	471	5′-CCT-GTC-CCA-GAG-GAG-GGC-3′
VCAM-1 antisense	471	5′-CAA-CTG-CGA-GCC-GAC-TTC-G-3′
P-selectin sense	427	5′-CGG-GGC-TTC-AGG-ACA-ATG-G-3′
P-selectin antisense	427	5′-TGG-GTC-ATA-TGC-AGC-GTT-CG-3′
E-selectin sense	362	5′-GCA-CCA-CAG-CGA-GGC-CAC-3′
E-selectin antisense	362	5′-CGG-GGA-GGG-TTG-GCT-GGG-3′
KC sense	476	5′-ATG-GTC-TCA-GCC-ACC-CGC-T-3′
KC antisense	476	5′-CGA-TGA-AAC-GCA-TCC-ACA-TCA-3′
IP-10 sense	357	5′-AAC-TGT-CCC-TGT-TTC-TCC-TG-3′
IP10 antisense	357	5′-GGA-AGG-TGG-TGG-TAA-GTT-TG3′
MIP-2 sense	329	5′-GCT-CCT-CAA-TGC-TGT-ACT-GG-3′
MIP-2 antisense	329	5′-GAT-TCT-GCC-CGT-TGA-GGT-AC-3′
MCP-1 sense	440	5′-TGC-AGG-TCT-CTG-TCA-GGC-TT-3′
MCP-1 antisense	440	5′-CTC-TGT-CAT-ACT-GGT-CAC-TTC-3′
MIP-1α sense	275	5′-GGT-CTC-CAC-CAC-TGC-CCT-T-3′
MIP-1α antisense	275	5′-TCA-GGC-ATT-CAG-TTC-CAG-CTC-3′
IL-12 p40 sense	399	5′-AGC-ACC-AAA-CTA-CTC-CGG-AC-3′
IL-12 p40 antisense	399	5′GGA-GTG-CTC-CAG-GAG-TCA-G-3′