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. 2022 Dec 8;13:7582. doi: 10.1038/s41467-022-35256-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–c Bodyweight gain (Il18^fl/fl: n = 10; Il18^fl/flUcp1^Cre: n = 13), GTT and AUC of GTT (Il18^fl/fl: n = 9; Il18^fl/flUcp1^Cre: n = 12), ITT and AUC of ITT (Il18^fl/fl: n = 8; Il18^fl/flUcp1^Cre: n = 13) (a), EAT, SAT, BAT, and liver weights (Il18^fl/fl: n = 7; Il18^fl/flUcp1^Cre: n = 10) (b), and energy intake (Il18^fl/fl: n = 10; Il18^fl/flUcp1^Cre: n = 11) (c) in Il18^fl/fl and Il18^fl/flUcp1^Cre mice fed a HFD for 12 weeks. d–e H&E (Il18^fl/fl: n = 11; Il18^fl/flUcp1^Cre: n = 9) (d) and UCP1 immunostaining (Il18^fl/fl: n = 23; Il18^fl/flUcp1^Cre: n = 23) (e) of BAT from indicated groups of mice. Scale: 50 μm. Inset: 25 μm. f RT-PCR analysis of thermogenic, lipolytic, and mitochondrial genes expression in BAT from indicated mice (Il18^fl/fl: n = 8; Il18^fl/flUcp1^Cre: n = 11). g Immunoblots and quantification of UCP1, PGC1α, and Cyt C relative to GAPDH in BAT from indicated mice (n = 6 per group). h-i. H&E (h) and UCP1 immunostaining (i) of BAT from indicated mice (n = 7 per group). Scale: 50 μm. Inset: 25 μm. j. Immunoblots and quantification of UCP1 and Cyt C relative to GAPDH in BAT from indicated mice (n = 6 per group). k RT-PCR analysis of thermogenic, lipolytic, and mitochondrial genes in BAT from indicated mice (n = 8 per group). Data are mean ± SEM, two-way ANOVA repeated-measures, followed by LSD post-test (a), two-sided Student’s t-test (b-c, e-k), and two-sided Mann-Whitney U test (d). Sample sizes were all biologically independent samples.