Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 8;13:7582. doi: 10.1038/s41467-022-35256-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — a–c Mouse metabolic parameters, including VO₂ (a), VCO₂ (b), and energy expenditure (c) and their average values from full day cycle, light cycle, and dark cycle during 48 hrs of monitoring in Ncc^fl/fl, Ncc^fl/flUcp1^Cre mice after 7 days of CL316243 treatment (Ncc^fl/fl: n = 7; Ncc^fl/flUcp1^Cre: n = 6). d–e Representative images of H&E and UCP1 immunostaining of BAT sections and quantification of adipocyte size and UCP1-positive area in EAT, SAT, and BAT from indicated groups of mice (Ncc^fl/fl: n = 7; Ncc^fl/flUcp1^Cre: n = 6). Scale: 50 μm. Inset: 25 μm. f RT-PCR analysis of thermogenic, lipolytic, and mitochondrial genes in EAT, SAT, and BAT from indicated mice (Ncc^fl/fl: n = 7; Ncc^fl/flUcp1^Cre: n = 6). g Immunoblots and quantification of UCP1 and PGC1α relative to GAPDH in BAT from indicated mice. n = 6 per group. Data are mean ± SEM, two-sided Student’s t-test (a–c, d, f) and two-sided Mann-Whitney U test (e). Sample sizes were all biologically independent samples.