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. 2022 Dec 8;13:7582. doi: 10.1038/s41467-022-35256-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a–c Bodyweight gain (Il18r^fl/fl: n = 8; Il18r^fl/flAdipoq^Cre: n = 9), GTT and AUC of GTT (n = 8 per group), ITT and AUC of ITT (n = 8 per group) (a), EAT, SAT, BAT, and liver weight (Il18r^fl/fl: n = 8; Il18r^fl/flAdipoq^Cre: n = 9) (b), and energy intake (n = 7~8 each) (Il18r^fl/fl: n = 7; Il18r^fl/flAdipoq^Cre: : n = 9) (c) in Il18r^fl/fl and Il18r^fl/flAdipoq^Cre fed a HFD for 12 weeks. d H&E of EAT, SAT, and BAT from indicated groups of mice (Il18r^fl/fl-EAT: n = 9; Il18r^fl/flAdipoq^Cre-EAT: n = 9; Il18r^fl/fl-SAT: n = 12; Il18r^fl/flAdipoq^Cre-SAT: n = 9; Il18r^fl/fl-BAT: n = 11; Il18r^fl/flAdipoq^Cre-BAT: n = 8). Scale: 50 μm. Inset: 25 μm. e–f RT-PCR analysis of lipogenic, lipolytic, glucose metabolic, thermogenic, and inflammatory genes in EAT (e) and SAT (f) from indicated mice (Il18r^fl/fl: n = 10; Il18r^fl/flAdipoq^Cre: n = 9). g Immunoblots of pAKT, AKT, and GAPDH and quantification of pAKT/AKT in WAT from indicated mice (n = 6 per group). h-i. Representative FACS images and quantification of CD11b⁺Siglec F⁺ eosinophils (Il18r^fl/fl-EAT: n = 8; Il18r^fl/flAdipoq^Cre-EAT: n = 6; Il18r^fl/fl-SAT: n = 9; Il18r^fl/flAdipoq^Cre-SAT: n = 6; Il18r^fl/fl-BAT: n = 4; Il18r^fl/flAdipoq^Cre-BAT: n = 4) (h) and CD11b⁺F4/80 ⁺ total, CD11b⁺F4/80 ⁺CD11c⁺ M1, and CD11b⁺F4/80 ⁺CD206⁺ M2 macrophages in EAT, SAT, and BAT (Il18r^fl/fl-EAT: n = 6; Il18r^fl/flAdipoq^Cre-EAT: n = 6; Il18r^fl/fl-SAT: n = 7; Il18r^fl/flAdipoq^Cre-SAT: n = 6; Il18r^fl/fl-BAT: n = 4; Il18r^fl/flAdipoq^Cre-BAT: n = 3) (i) from indicated mice. Data are mean ± SEM, two-way ANOVA repeated-measures, followed by LSD post-test (a), two-sided Student’s t-test (b–d, h) and two-sided Mann-Whitney U test (g). Sample sizes were all biologically independent samples.