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. 2022 Nov 25;13:981581. doi: 10.3389/fpls.2022.981581

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Copyright © 2022 Daems, Ceusters, Valcke and Ceusters

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chlorophyll a fluorescence OJIP transient curves measured at 08.00 h in young fully expanded leaves of Phalaenopsis ‘Edessa’ at 0 (control), 1, 8, 15 and 22 days after exposure to chilling conditions (10°C). Transients were double normalized between O and P steps: V_t (FvF) = (F_t – F₀)/(F_M – F₀) and plotted on a logarithmic time scale (A). Plotted relative variable fluorescence V_t(chilling) – V_t(control) for visualization of J-band (ΔV_J) and I-band amplitudes (ΔV_I) on a logarithmic time scale (B). Changes in O-K phase relative variable fluorescence intensity (ΔW_OK, L-band) (C), O-J phase relative variable fluorescence intensity (ΔW_OJ, K-band) (D) and in O-I phase relative variable fluorescence intensity (ΔW_OI) (E). Averaged transients from each time point are illustrated (n = 15 plants). The zero line represents the control group in B, C, D, and E.