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. 2022 Nov 23;25(12):105662. doi: 10.1016/j.isci.2022.105662

Figure 2.

Figure 2

S100A8 attenuated LPS-induced inflammation via TLR4

TLR4 knockout (TLR4KO) mice or wild-type (WT) mice at 12 weeks of age were used for experiments.

(A) Peritoneal macrophages from the indicated mice were incubated with PBS, LPS (2 μg/mL), or S100A8 (0.3 μg/mL) for 2 h and then analyzed by qPCR. mRNA expression of the indicated inflammatory cytokine and S100 genes in peritoneal macrophages (n = 3).

(B) Peritoneal macrophages from the indicated mice were pretreated with PBS or S100A8 (0.3 μg/mL) for 30 min before LPS (2 μg/mL) stimulation for 2 h and then analyzed by qPCR. mRNA expression of the indicated inflammatory cytokine and S100 genes in peritoneal macrophages (n = 3).

All data from three independent experiments are represented as the mean ± SEM ∗p < 0.05, ∗∗p < 0.01.