Figure 3.

Peritoneal macrophages and peritoneal cells from mice with obesity and diabetes exhibited insufficient production of S100A8 after LPS treatment

db/db and db/+ mice or ob/ob and wild-type (WT) mice at 8 weeks of age were used for experiments (A–M).

(A) Body weight of db/db and db/+ mice (n = 10).

(B) Casual blood glucose levels of db/db and db/+ mice (n = 10).

(C) Body weight of ob/ob and WT mice (n = 10).

(D) Casual blood glucose levels of ob/ob and WT mice (n = 10).

(E) Experimental protocol for an in vivo study. Mice were injected with PBS or LPS (12.5 μg/gBW), and serum was collected at 4 h after injection.

(F) Serum S100A8 levels in db/db and db/+ mice (n = 4).

(G) Serum S100A8 levels in ob/ob and WT mice (n = 4).

(H) Experimental protocol for an in vivo study. Mice were injected with PBS or LPS (12.5 μg/gBW), and peritoneal cells were analyzed by qPCR at 4 h after injection.

(I) mRNA expression of S100a8 in peritoneal cells from db/db or db/+ mice (n = 4).

(J) mRNA expression of S100a8 in peritoneal cells from ob/ob or WT mice (n = 4).

(K) Experimental outline of an in vitro study. Peritoneal macrophages were incubated with PBS or LPS (2 μg/mL) for 2 h and analyzed by qPCR.

(L) mRNA expression of S100a8 in peritoneal macrophages from db/db or db/+ mice (n = 4).

(M) mRNA expression of S100a8 in peritoneal macrophages from ob/ob or WT mice (n = 4).

C57BL/6J mice fed a Western diet (WD) or normal diet (ND) from 8 to 16 weeks of age were used for experiments (N-R).

(N) Body weight and (O) casual blood glucose levels (n = 20-30).

(P) mRNA expression of the indicated inflammatory cytokine and S100 genes in peritoneal cells (n = 7).

(Q) mRNA expression of the indicated inflammatory cytokine and S100 genes in peritoneal cells at 4 h after PBS or LPS injection (12.5 μg/gBW) (n = 4).

(R) Serum S100A8 levels at 4 h after PBS or LPS (12.5 μg/gBW) injection (n = 4).

All data from three independent experiments are represented as the mean ± SEM ∗p < 0.05, ∗∗p < 0.01.