Table 3.
An example of depositing indexed P5 and P7 PCR primers in sub-library generation
| P7 primers (a total of 96) used for all sub-libraries | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Plate | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | P7_#1 | P7_#2 | P7_#3 | P7_#4 | P7_#5 | P7_#6 | P7_#7 | P7_#8 | P7_#9 | P7_#10 | P7_#11 | P7_#12 |
| B | P7_#13 | P7_#14 | P7_#15 | P7_#16 | P7_#17 | P7_#18 | P7_#19 | P7_#20 | P7_#21 | P7_#22 | P7_#23 | P7_#24 |
| … | …... | |||||||||||
| H | P7_#85 | P7_#86 | P7_#87 | P7_#88 | P7_#89 | P7_#90 | P7_#91 | P7_#92 | P7_#93 | P7_#94 | P7_#95 | P7_#96 |
| One unique P5 primer added to all 96 wells of each sub-library | ||||||||||||
| Sub-library #1 | P5_#1 | |||||||||||
| Sub-library #2 | P5_#2 | |||||||||||
| Sub-library #3 | P5_#3 | |||||||||||
| Sub-library #4 | P5_#4 | |||||||||||
| … | …... | |||||||||||