Annealing Buffer

Reagent	Final concentration	Amount
Tris-HCl pH 8.0 (1 M)	40 mM	400 μL
NaCl (5 M)	50 mM	100 μL
Nuclease-free H2O	N/A	9.5 mL
Total	N/A	10 mL

Annealing Buffer can be stored at 4°C for at least 6 months.