Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Tagmentation Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl pH 7.5 (1 M) | 20 mM | 200 μL |
| MgCl2 (1 M) | 10 mM | 100 μL |
| Dimethylformamide | 20% (v/v) | 2 mL |
| Nuclease-free H2O | N/A | 7.7 mL |
| Total | N/A | 10 mL |
Tagmentation Buffer can be stored at −20°C for at least 12 months.
