Table 1.
Tracking parameters for MatlabTrack
| Interval (ms) | Max frame to frame displacement | Nmin | Max end to end displacement |
|---|---|---|---|
| 15 | 0.31 | 22 | 0.73 |
| 200 | 0.43 | 6 | 0.61 |
| 500 | 0.47 | 4 | 0.65 |
| 1,000 | 0.55 | 4 | 0.67 |
Based on the time interval for imaging, appropriate tracking parameters needs to be filled in for merging data from all the movies and preparing the survival time distribution. We have quantified these parameters by tracking histone H3 (Hht1) in yeast S. cerevisiae.