FIG. 2.

Inhibitory effects of various peptides on the uptake of FITC-conjugated E. coli O111:B4 LPS by CHO-CD14 cells (left side of figure) and by human PBMC (right side of figure) in the presence of different concentrations of NHS. FITC-conjugated LPS (1 μg/ml) was preincubated with peptides, in the presence or absence of NHS, at 37°C for 15 min, and the mixture was then added to cells and incubated at 37°C for an additional 30 min. Cell-associated fluorescence was measured by flow cytometry, and the data are expressed as the MFI. Panels A to D document the influence of PMB, SAEP-4, SAEP-2, and “nonsense peptide,” respectively, on the cell uptake of LPS. The P value shown in parentheses below each horizontal axis indicates the statistical significance, determined by two-way ANOVA, of differences in MFI produced by the material specified for that axis. N.S., not significant (P > 0.05).