FIGURE 6.

Truncation of a PEST‐like sequence alters the distribution of MLPH in H1299 cells. (a) H1299 cells transfected with WT DsRed_MLPH (left) or DsRed_MLPH Δ390‐415 (right) were analyzed by immunofluorescence of FITC_ACTIN. Cells were treated with 0 mM Ca²⁺ or 10 μM Calpain inhibitor III. White arrows in the panels point to puncta formed by MLPH aggregates. FITC phalloidin staining for visualization of the actin cytoskeleton; blue arrows point to actin aggregates in the cell cortex. (b) H1299 cells transfected with WT DsRed_MLPH (left) or DsRed_MLPH Δ390‐415 (right) were cotransfected with PD‐L1_EGFP. Cells were treated with 0 mM Ca²⁺ or 10 μM Calpain inhibitor III. White arrows in the panels point to puncta formed by MLPH aggregates. For all of Figure 6, data are representative of at least three independent experiments.