TABLE 1.

Information of primers, plasmids, and antibodies

Name	Sequence
pHluorinCD63‐PDL1‐SP_F	5’‐AGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCACTAGCCACCATGGGAAGTAAAGG‐3’
pHluorinCD63_R	5’‐GCTGCTTCCACCTCCACCGCTACCGCTACCGCTACCTCGAGCTCCACCGGTTTTG‐3’
pHluorinCD63‐PDL1_NF	5'‐ACGCTTTGCCGCCACCATGAGGATATTTGCTGTCTTTATATTCATG‐3’
pHluorinCD63‐PDL1_NR	5'‐GCTGCTTCCACCTCCACC‐3'
pHR_PD‐L1‐CLIP‐C_F	5’‐GGTAGCGGTAGCGGTAGC‐3’
pHR_PD‐L1‐CLIP‐C‐His_R	5’‐GGGTTTAAACTCAATGGTGATGGTGATGATGACCGGTCGTCTCCTCCAAATGTGTATCAC‐3’
pHR_PD‐L1‐pHluorin_F	5’‐AGATCCGCTAGCGCCACCATGAGGATATTTG‐3’
pHR_PD‐L1‐pHluorin‐His_R	5’‐AACCTAGAATTCTCAATGGTGATGGTGATGATG‐3’
MLPH_HindIII‐390_F	5’‐GAGCTCAAGCTTCGAATTCAATGGGGAAGA‐3’
MLPH_HindIII‐390_R	5’‐AACTGATTTGTCCCTGTTCAGCTTCCTCCTCAGGGC‐3’
MLPH_415‐BamHI_F	5’‐GCCCTGAGGAGGAAGCTGAACAGGGACAAATCAGTT‐3’
MLPH_415‐BamHI_R	5’‐GTGGATCCTTAGGACTGGTGGGCCACCACA‐3’
Slp2‐a_T614*_F	5’‐TAAAGGAAATATTCAGTTTGCAATTGAATAGGTGGAGTCACTGAAGG‐3’
Slp2‐a_T614*_R	5’‐CCTTCAGTGACTCCACCTATTCAATTGCAAACTGAATATTTCCTTTA‐3’
H‐ORAI1_F	5’‐AGGTGATGAGCCTCAACGAG‐3’
H‐ORAI1_R	5’‐CTGATCATGAGCGCAAACAG‐3’
M‐ORAI1_F	5’‐ CATAAGACGGACCGGCAGTT‐3’
M‐ORAI1_R	5’‐ AGGAAGAGGTGTCCCTTGTG‐3’
H‐Slp2‐a_R	5’‐TCTTCTACAACGCCAGCCAG‐3’
H‐Slp2‐a_F	5’‐ AGGGCCGAAGAAGAGAGAGT‐3’
H‐Mlph_F	5’‐AAGAGGTTCGGAAGTGCCAAGG‐3’
H‐Mlph_R	5’‐ AGCCAGGTTCTCCATCCTCATC‐3’

Name	Resource	Note
pCMV‐CD63‐pHluorin	Tom Martin at University of Wisconsin‐Madison
pHR‐CLIP‐PD‐L1	Enfu Hui at UC San Diego
pLJM1_FLAG	Zheng Zhang at Xiangya School of Medicine, Central South University
pLV[shRNA]‐Scamblem	Vectorbuilder	GCTTCGCGCCGTAGTCTTA
pLV[shRNA]‐hOrai1	Vectorbuilder	GGTGAGCAACGTGCACAATCT
pLV[shRNA]‐mOrai1	Vectorbuilder	TCTGCTGGGTCAAGTTCTTAC
pLV[shRNA]‐hMlph	Vectorbuilder	GTCTTCTGAGAGTCAGATCTT
pLV[shRNA]‐hSlp2‐a	Vectorbuilder	GCCTTGATCTACCACTGCTAA
pMD2.G	Addgene #12259
pMDLg/pRRE	Addgene #12251
pRSV‐Rev	Addgene #12253
DsRed_MLPH	Addgene #89231

Antibody	Company	Cat. No.	ST con.
Anti‐Orai1 Antibody	Abcam, Cambridge, UK	ab175040	1:1000
Anti‐CD63(E‐12) Antibody	Santa Cruz, TX, USA	sc‐365604	1:1000
Anti‐HRS(D7T5N) Antibody	Cell Signaling Technology, MA, USA	15087	1:1000
Anti‐PD‐L1(E1L3N) Antibody	Cell Signaling Technology, MA, USA	13684	1:1000
Anti‐CD63 Antibody (MX‐49.129.5)	Santa Cruz, TX, USA	sc‐5275	1:1000
Anti‐PD‐L1 Antibody	Proteintech Group, IL, USA	66248‐1‐Ig	1:1000
Anti‐SLP2 Antibody	Proteintech Group, IL, USA	12359‐1‐AP	1:1000
Anti‐Slac2‐a (G‐10) Antibody	Santa Cruz, TX, USA	sc‐515894	1:1000
Anti‐Histone H3 antibody	Abcam, Cambridge, UK	ab201456	1:2000
Anti‐Annexin A1/ANXA1 Antibody	Abcam, Cambridge, UK	ab214486	1:1000
Anti‐GM130 Antibody	Abcam, Cambridge, UK	ab52649	1:1000
Anti‐GAPDH	Santa Cruz, TX, USA	sc‐32233	1:1000
Anti‐β‐actin	Santa Cruz, TX, USA	sc‐47778	1:2000
Anti‐Rabbit HRP‐Conjugated Secondary Antibodies	Abmart, Shanghai, China	M21002	1:4000
Anti‐Mouse HRP‐Conjugated Secondary Antibodies	Abmart, Shanghai, China	M21001	1:4000
Animal experiments
GoInVivoTM Purified anti‐mouse CD274 Antibody	BioLegend, San Diego, CA, USA	124328
GoInVivoTM Purified Rat IgG2b, κ Isotype Ctrl Antibody	BioLegend, San Diego, CA, USA	400666
Flow cytometry analysis
Anti‐Mouse CD16/32 Antibody	BioLegend, San Diego, CA, USA	101303
FITC Anti‐Mouse CD45 Antibody	BioLegend, San Diego, CA, USA	103108
Percp/Cyanine5.5 Anti‐Mouse CD3 Antibody	BioLegend, San Diego, CA, USA	100218
APC Anti‐Mouse CD4 Antibody	BioLegend, San Diego, CA, USA	100412
PE/Cyanine7 Anti‐Mouse CD8a Antibody	BioLegend, San Diego, CA, USA	100722
PE Anti‐Mouse CD366 (Tim‐3) Antibody	BioLegend, San Diego, CA, USA	134003
PE/Dazzletm 594 Anti‐Mouse CD279 (PD‐1) Antibody	BioLegend, San Diego, CA, USA	135228
PE Anti‐Human/Mouse Granzyme B Recombinant Antibody	BioLegend, San Diego, CA, USA	396406
PE/Dazzletm 594 Anti‐Mouse Ki‐67 Antibody	BioLegend, San Diego, CA, USA	652428