Table 1.
Antibodies utilized for flow cytometry
Antigen | Conjugate | Clone | Source | Catalogue | Dilution |
---|---|---|---|---|---|
AREG | Biotin—strepBV711 | BAF989 | Roche | BAF989 | 1:50 |
BATF | PE | MBM7C7 | Thermo Fisher Scientific | 12-9860-42 | 1:50 |
CD11b | APC-eFluor780 | M1/70 | eBioscience | 47-0112-82 | 1:800 |
CD19 | APC-eFluor780 | 1D3 | eBioscience | 47-0193-82 | 1:400 |
CD27 | PE/Dazzle594 | LG.3A10 | BioLegend | 124228 | 1:400 |
CD27 | BV510 | LG.3A10 | BD | 563605 | 1:200 |
CD3 | BV650 | 17A2 | BioLegend | 100229 | 1:200 |
CD30L | PE | RM153 | BioLegend | 106405 | 1:200 |
CD4 | APC-eFluor780 | GK1.5 | Invitrogen | 47-0041-82 | 1:200 |
CD44 | PerCP-Cy5.5 | IM7 | BioLegend | 103032 | 1:100 |
CD8A | APC-eFluor780 | 53-6.7 | Invitrogen | 47-0081-82 | 1:100 |
CD8A | BUV805 | 53-6.7 | BD | 564920 | 1:200 |
CXCR6 | BV711 | SA051D1 | BioLegend | 151111 | 1:200 |
DAPI | N/A | N/A | Sigma-Aldrich | D9542 | 1 mg/ml |
EpCAM | APC-eFluor780 | G8.8 | eBioscience | 47-5791-82 | 1:50 |
Phospho-FOXO1 | N/A | Cell Signaling | 9464 | 1:50 | |
ICOS | PE-Cy7 | 7E.17G9 | Thermo Fisher Scientific | 313519 | 1:50 |
IFNγ | PE-Cy7 | XMG1.2 | eBioscience | 25-7311-82 | 1:200 |
IL-17A | PE | eBio17B7 | eBioscience | 12-7177-81 | 1:200 |
IL-17A | APC | eBio17B7 | eBioscience | 17-7177-81 | 1:200 |
IL-17F | PerCP-Cy5.5 | 079-289 | BioLegend | 562194 | 1:100 |
IRF4 | PE-Cy7 | IRF4.3E4 | BioLegend | 646413 | 1:100 |
JAML | A647 | 4E10 | BioLegend | 128506 | 1:50 |
NK1.1 | BV421 | PK136 | BioLegend | 108731 | 1:100 |
NKG2D | PE/Dazzle594 | CX5 | BioLegend | 130214 | 1:100 |
NKG2D | APC | CX5 | eBioscience | 17-5882-82 | 1:200 |
PD-1 | BV510 | 29F.1A12 | BioLegend | 135241 | 1:100 |
PD-1 | PE-Cy7 | 29F.1A12 | BioLegend | 135215 | 1:100 |
RANKL | PE | IK22/5 | BioLegend | 510005 | 1:25 |
RORγt | PE-Cy7 | B2D | Thermo Fisher Scientific | 25-6981-82 | 1:100 |
SCA-1 | BV605 | D7 | BioLegend | 108133 | 1:100 |
Streptavidin | BV711 | N/A | BioLegend | 405241 | 1:100 |
TCR Vγ1 | PE | 2.11 | Biolegend | 141106 | 1:200 |
TCR Vγ4 | APC | UC3-10A6 | BioLegend | 137707 | 1:100 |
TCR Vγ6 | Pacific Blue | 1C10-1F7 | Hatano et al., 2019 | N/A | 1:50 |
TCRδ | FITC | GL3 | eBioscience | 11-5711-85 | 1:200 |
TIM-3 | PE/Dazzle594 | B8.2C12 | BioLegend | 134013 | 1:200 |
Zombie NIR | N/A | N/A | BioLegend | 423106 | 1:400 |
Anti-rabbit IgG-biotin | N/A | Sigma-Aldrich | SAB4600006 | 1:1,000 |