FIG. 4.

Effect of salt concentration on the bactericidal activity of LL-37/hCAP18. ChoP-on (H512; solid bars) and ChoP-off (H445; stippled bars) constitutive mutants of strain Eagan were treated for 60 min at 37°C with LL-37/hCAP18 (final concentration, 5 μg/ml), and the number of survivors was determined in viable counts. NaCl was added to medium E (16.7 mM Na⁺) in bactericidal assays at the final concentrations indicated at the bottom. Results are expressed in relation to the same conditions without peptide and are the mean of two determinations in duplicate.