FIG. 5.

Binding of LL-37/hCAP18 to H. influenzae Rd with and without LPS-ChoP. Equivalent numbers of constitutive ChoP-off strain H446 (A) or ChoP-on strain H491 (B) were incubated with the concentration (in μg/ml) of LL-37/hCAP18 indicated above each lane. After removal of unbound peptide, the binding of LL-37/hCAP18 to the bacteria was determined in whole cell lysates. Following separation on SDS–18% polyacrylamide gels, the amount of bound peptide was determined by Western blotting using an antiserum raised against LL-37/hCAP18.