FIG. 7.
Detection of LL-37/hCAP18 mRNA in H. influenzae-infected Detroit 562 pharyngeal carcinoma cells in culture. RNA was isolated from uninfected cells (lanes 1 and 3) or cells infected for 4 h with 106 CFU of strain H491 per ml (lanes 2 and 4) and subjected to RT-PCR using primers specific to the gene for LL-37/hCAP18 (lanes 1 and 2). RT-PCR using primers to the glyceraldehyde 3-phosphate dehydrogenase gene served as controls for the quality of the template (lanes 3 and 4). The PCR products were visualized using ethidium bromide after separation on a agarose gel. Molecular weight markers consisted of a 100-bp ladder.