Transmission electron micrographs of B. pseudomallei-A. astronyxis coculture. (a) Pseudopodium in contact with polar end of bacillus shortly after start of coculture (magnification, ×18,700); (b) membrane-lined vacuoles containing single bacillus inside amebic trophozoite, surrounded by multilamellate membrane (×18,700); (c) membrane-lined vacuole containing multiple bacilli apposed to external trophozoite surface (×12,800); (d) multiple bacterium-laden vacuoles formed after 60 min of coculture (×8,000).