Figure 1.

The nuclear pore complex (NPC) linker scaffold. (A) Schematic cutaway representation of the NPC architecture. (B) Near-atomic composite structure of the human NPC symmetric core generated by quantitatively docking nucleoporin and nucleoporin complex crystal and single particle cryo–electron microscopy (cryo-EM) structures into an ∼12-Å cryo–electron tomographic (cryo-ET) map of the intact human NPC (Mosalaganti et al. 2022; Petrovic et al. 2022). The nuclear envelope is shown as a gray isosurface and the nucleoporin structures are displayed in cartoon representation colored according to the legend. (C) Schematic layer-by-layer representation of a cross-section view of three spokes of the NPC inner ring showing the network of linker–scaffold interactions. (D) Schematic representation of the top views of human NPC cytoplasmic and nuclear outer rings and corresponding side views of two spokes, illustrating the reticulated head-to-tail arrangement of the coat nucleoporin complexes (CNCs) cross-linked by trans-spoke NUP93–NUP205 interactions. (Figure adapted from Petrovic et al. 2022, with permission from the authors.)