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. 2022 Dec 8;23:816. doi: 10.1186/s12864-022-09050-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — UpSet plots, summarising unique and shared differentially expressed genes due to exposure to different treatments. Black dots in panel matrices correspond to different Venn diagram sections: connected dots indicate intersections of sets of differentially expressed genes; singular dots represent unique sets of differentially expressed genes. Vertical barplots summarise the number of upregulated (a) and downregulated (b) genes. Horizontal bars indicate the total number of regulated genes in each treatment combination. The set union of all upregulated and downregulated genes detected in salinity treatments represents 443 salinity genes, of which one gene was upregulated when increased salinity was combined with reduced flow but downregulated when all three stressors were applied (= 442 exclusively regulated genes)