Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 9;20:578. doi: 10.1186/s12967-022-03794-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 7 — Up-regulation of CD8⁺ T cells surface markers CD69 (early), CD71 (middle) and CD25 (late) by Stx1B-scFv OKT3 lectibody. Gb3⁺ Ramos or Gb3⁻ Namalwa cells were co-cultured with PBMCs in the effector-to-target (E:T) ratio of 5:1 and purified Stx1B-scFv OKT3 lectibody (35.6 nM). Mean Fluorescence Intensity (MFI) data from three different donors were analyzed in flow cytometry from live CD8⁺ gated PBMCs. Results are expressed as a mean ± SD (n = 3) from separate experiments. After 24 h, the surface expression of T cell activation surface markers a CD69 and b CD71 was determined. c CD71 and d CD25 surface expression was monitored at 48 h following co-incubation of target and effector cells in presence of lectibody. CD8⁺ T cells were significantly activated when co-incubated with Gb3-expressing tumor cells and Stx1B-scFv OKT3, while they remained in a resting state when treated with Gb3-negative cells and lectibody. Statistical differences were determined with a two-tailed, unpaired t-test between control and each treatment group. Tests with a p-value ≤ 0.05 are considered statistically significant and marked with an asterisk (*), and p-values ≤ 0.01 are shown as two asterisks (**)