Fig. 3.

Cell cycle phase distribution. For cell cycle phase analyses 10 Gy was delivered to mice bearing NSC11 or NSC20 tumors 40 days post-implant with tumors collected at specified time points. A Cell cycle phase distribution of GFP expressing cells as defined using flow cytometry. Bars represent the percentage of NSC11 and NSC20 GSCs in each cell cycle phase in OB and RH. Distributions represent the mean ± SEM for 3–4 mice. B Immunohistochemical determination of the percentage of tumor cells expressing the G2 marker CENPF with tumor cells identified by Sox2 staining. Values represent the mean ± SEM for 3–4 mice. C Immunohistochemical determination of the percent tumor cells expressing the mitosis marker phospho-H3 with tumor cells identified by Sox2 staining. Values represent the mean ± SEM for 3–4 mice. *p-val < 0.05