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Concentration–effect assessment of 16d against Spns2 and sphingosine kinases. S1P release decreased as a function of increased concentrations of 16d in Spn2-transfected Hela cells (A). Inhibition of recombinant mSphK1 (B) and mSphK2 (C) inhibition using a standard TLC-based assay. The assays were performed in duplicate.
