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Pharmacodynamic and pharmacokinetic analysis of 16d in rats following a 10 mg/kg IP injection. All rats were 4 week old (Sprague-Dawley strain, n = 4) males. (A) Levels of 16d in blood. (B) Blood lymphocyte concentrations reached a minimum 4 h after treatment with 16d. Lymphocyte counts were determined using a Heska Element HT5 blood analyzer, while 16d levels were determined by LC/MS using 57e as an internal standard.
