Table 2.

Spns2 Inhibitory Activity of the Tail and Homologation of the Linker Region^a

Cmpd	R	% Inhibition	Cmpd	R	% Inhibition
52a		34 ± 8	62a		49 ± 3
52b		40 ± 5	62b		50 ± 2
57a		55 ± 7	62c		24 ± 3
57b		52 ± 4	62d		8 ± 0
57c		70 ± 4	67		0 ± 14
57d		61 ± 4	72a		35 ± 5
57e		49 ± 1	72b		11 ± 6
57f		26 ± 4

^aSpns2 inhibition is presented as percent inhibition relative to the control, during which no inhibitor is introduced. All compounds were assayed with a 2 μM inhibitor. Cell media were extracted, and S1P concentrations were measured by LC/MS. Compound measurements were performed in duplicate.