Table 3.

Spns2 Inhibitory Activity of Heteroaromatic Linkers^a

Cmpd	R	% Inhibition	Cmpd	R	% Inhibition
28		45 ± 4	38		45 ± 4
30		49 ± 4	40		44 ± 5
32		63 ± 3	42		50 ± 4
34		51 ± 3	47		59 ± 3

^aSpns2 inhibition is presented as a percent inhibition relative to the control, during which no inhibitor is introduced. All compounds were assayed with a 2 μM inhibitor. Cell media were extracted, and S1P concentrations were measured by LCMS. Compound measurements were performed in duplicate.