Figure 1. Human DSCC1-RFC has roles in sister chromatid cohesion, DNA replication, and DNA repair.

(A) SCC defects of indicated cell lines. At least 50 metaphases were scored per condition; three independent experiments are shown as separate bars. (B) DNA replication fork speed of indicated cell lines was assessed with a DNA fiber assay. At least 65 fibers were scored per experiment per condition in two independent experiments. Indicated P-value is calculated with an unpaired t test. Scale bar represents 5 µm. (C) Cells were assessed for γH2AX foci using immunofluorescence. At least 47 cells were scored per experiment per condition in three independent experiments, and the percentage of cells with more than five foci is shown. The P-value is calculated with an unpaired t test. Scale bar represents 5 µm. (D) WT and DSCC1-KO cells were treated with indicated drugs and proliferation relative to untreated cells was assessed after 5 d by a CTB assay. Three technical replicates from a representative of two independent experiments are shown.

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