Figure 2. Genome-wide CRISPR screen reveals multiple synthetic lethal interactions of DSCC1-KO cells.

(A) Network of hits synthetically sick/lethal with DSCC1-KO as determined by DrugZ analysis with an FDR < 0.1. Edges indicate physical protein–protein interactions as annotated in Genemania.org. (B) Gene Ontology enrichment of <0.1 FDR hits synthetically sick/lethal with DSCC1-KO extracted from String-db. (C) WT and DSCC1-KO cells were transfected with indicated crRNAs and treated with doxycycline to induce Cas9 expression. After 7 d, cell viability was assessed by CTB assay and normalized for cells depleted of the nonessential olfactory receptor OR10A7. POLR2L was included as a common essential control. Dots indicate means calculated from three technical replicates of three independent experiments. (D) Clonogenic survival of WT and DSCC1-KO cells 11 d after crRNA transfection and Cas9 induction. Figure shows a representative of two independent experiments.

Source data are available for this figure.