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. 2022 Dec 8;6(2):e202201533. doi: 10.26508/lsa.202201533

Figure 2. SLAMF6 and TCR signaling complexes share key mediators.

Figure 2.

V5-SLAMF6-expressing Jurkat T cells were stimulated with plate-coated αCD3 + αSLAMF6 (plate) or plate-coated αCD3 + soluble αSLAMF6 (soluble) for 15 min. The cells were then lysed and the lysate mixed with V5-coupled agarose beads to enrich for V5-tagged SLAMF6 immunoprecipitation. Pull-down lysate proteins were separated by electrophoresis and submitted for mass spectrometry analysis. (A) Protein enrichment pathway analysis for the plate versus soluble stimulation conditions was performed. (B) Proteins interacting with SLAMF6 were identified, listed by peptide-spectrum match score. (C) A schematic demonstrating that the SLAMF6-interacting proteins identified in the immunoprecipitation (marked by a purple star) are known to be essential for proximal TCR signal transduction, emphasizing the interconnection between the two receptors and their signaling interactome. Three independent experimental repeats were performed. (n = 3).