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. 2022 Dec 8;6(2):e202201533. doi: 10.26508/lsa.202201533

Figure 3. SLAMF6 clustering in the immunologic synapse (IS) enhances cytokine secretion.

Figure 3.

(A) A schematic representation of SLAMF6 in the absence (top) or presence (bottom) of homophilic receptor ligation in a T-B cell co-culture. (B) Jurkat T cells were transfected via nucleofection with LifeAct mCherry and SLAMF6 GFP, then co-cultured with Raji B Cell APCs. Images are representative of at least 30 cells from a least two independent experiments. Scale bar is 5 μm. Actin clearance was defined as a mature IS. (C) Jurkat T cells were treated with αCD3-conjugated beads and αSLAMF6-conjugated beads versus αCD3 + αSLAMF6–conjugated beads (both antibodies on the same bead). After 24 h, the supernatant was harvested and IL-2 levels were analyzed by ELISA for at least three independent experiments (n = 3). (D) Jurkat T cells were treated with αCD3 + αSLAMF6 or αCD3 + αSLAMF6 + cross-linker for 24 h, after which the supernatant was harvested and IL-2 levels were analyzed by ELISA for at least three independent experiments (n = 3). *P < 0.05 for an unpaired t test.