Figure S2. Protein–protein interactions downstream of SLAMF6 activation differ based on stimulation condition.

V5-SLAMF6-expressing Jurkat T cells were stimulated with plate-coated αCD3 + αSLAMF6 (plate) or plate-coated αCD3 + soluble αSLAMF6 (soluble) for 15 min. The cells were then lysed and the lysate mixed with V5-coupled agarose beads to enrich for V5-tagged SLAMF6 immunoprecipitation. Pull-down lysate proteins were separated by electrophoresis and submitted for mass spectrometry analysis. (A, B) Protein–protein interaction identified prediction models of kinases involved downstream of SLAMF6 signaling in the plate (A) and soluble (B) conditions. Kinases that were validated in the pull-down are shown in red.