Figure 1.

The transfer of MnT5H2 into tobacco. (A) Structure diagram of the pLGNL-MnT5H2 vectors. NPTII: neomycin phosphotransferase II gene. 35S: cauliflower mosaic virus 35S promoter. (B) Histochemical GUS staining of transgenic lines. (C) Kanamycin resistance gene NPTII positive identification of genome (+): pLGNL-MnT5H2 vector. (D) Quantitative real-time PCR analysis. WT, The wild tobacco; OE, The overexpressed transgenic plants. Data are means ± SDs (n=3). Different letters above the bars indicate the significant difference of different lines at P<0.05.