Figure 5.

FcRn affinity chromatography of the TM-YTE antibody panel. (a) As an example of how TM-YTE modifications affect FcRn binding, the elution profile of antibody 4 in the different Fc formats from an FcRn affinity column is shown. A later elution time indicates stronger FcRn binding. (b) FcRn binding of un-stressed, photo-stressed or heat-stressed antibodies was analyzed using FcRn affinity column. The retention time on the column was normalized between runs as described in the methods section to a well behaved YTE control antibody. A relative retention time of 1 would indicate equal FcRn binding to the control YTE antibody. (a) SEC chromatogram plotting absorbance at 280 nm versus time for Ab4 in WT, TM, YTE, and TM-YTE formats. TM and WT formats elute earlier compared to YTE and TM-YTE formats. (b) A bar graph plotting the relative retention time of Ab 1–8 after no stress, 7 day photo stress, or 14 day heat stress. No significant difference is observed between different conditions.