Table 2.

Heat-stress and photo-stress studies for TM-YTE SARS-COV-2 antibodies. Heat stress samples were formulated at 1 mg/mL in PBS, pH 7.2, and incubated at 45°C for 14 days. Photo-stress samples were formulated at 2.5 mg/mL in PBS, pH 7.2, and illuminated under 3,000 lux cool white light (CWL) for 7 days. Control samples were formulated at 1 mg/mL in PBS, pH 7.2, and incubated at 4°C. Changes in monomer, aggregate, and fragment content were then analyzed by HP-SEC compared to the control sample.

	Heat stress			Photo stress
Antibody	Change in monomer (%)	Change in aggregate (%)	Change in fragment (%)	Change in monomer (%)	Change in aggregate (%)	Change in fragment (%)
Antibody 1	−2.5	0.2	2.3	−1.0	0.8	0.2
Antibody 2	1.0	−2.1	1.1	−0.6	0.3	0.3
Antibody 3	−2.5	0.2	2.3	−0.8	0.6	0.2
Antibody 4	−1.2	0.5	0.7	−0.7	0.5	0.2
Antibody 5	−0.9	0.5	0.4	−1.0	1.0	0.0
Antibody 6	−0.8	0.1	0.7	−0.8	0.6	0.2
Antibody 7	−3.9	0.1	3.7	0.0	−0.2	0.3
Antibody 8	−0.2	−0.5	0.7	−0.5	0.3	0.1
NIP228 (WT Fc)	−0.7	0.0	0.7	−1.0	0.3	0.7
NIP228-TM	−0.4	0.0	0.4	−0.8	0.6	0.2
NIP228-YTE	−1.0	0.2	0.9	−0.6	0.4	0.1
NIP228-TM-YTE	−0.9	0.2	0.7	−0.6	0.6	0.0
Antibody 4 (WT Fc)	−1.7	0.0	1.7	−0.5	0.5	0.0
Antibody 7 (WT Fc)	−3.0	0.3	2.7	0.9	−0.9	0.0