Table 3.
Post-translational modification analysis of photo-stressed and heat-stressed TM-YTE SARS-COV-2 antibodies using peptide mapping by LC-MS. The antibody panel was heat stressed (45°C, 1 mg/ml, 14 days) or photo stressed (CWL, 2.5 mg/ml, 7 days) and then analyzed by reducing tryptic peptide mapping by LC-MS to monitor critical amino acid sites, as well as PTM increase in the Fc and Fv/CDR region.
| Photo stress |
Heat stress |
|||
|---|---|---|---|---|
| Antibody | % oxidation increase in M428 | % total oxidation increase in CDR | % total deamidation increase in CDR | % total isomerization increase in CDR |
| Antibody 1 | 2.2 | 0.4 | 0 | 3.0 |
| Antibody 2 | 2.3 | 0.3 | 0.2 | 3.2 |
| Antibody 3 | 1.5 | 0.3 | 0 | 4.5 |
| Antibody 4 | 1.3 | 2.0 | 0 | 0 |
| Antibody 5 | 1.7 | 0.2 | 7.8 | 1.0 |
| Antibody 6 | 1.4 | 5.4 | 3.1 | 6.0 |
| Antibody 7 | 1.2 | 0.9 | 0.2 | 13.8 |
| Antibody 8 | 2.0 | 1.1 | 12.9 | 7.8 |