Table 4.
Solution property assays of the TM-YTE antibody panel. The TM-YTE SARS-CoV-2 antibody panel was tested for RSA, solubility, and viscosity by AC-SINS, DLS, and a solubility/viscosity assay. NIP228 is a negative control antibody, and IL330396 is a positive control antibody.
| AC-SINS red shift (nm) | DLS KD (mL/gm) | Solubility (approximate feasible concentration; mg/mL) | Viscosity at 100 mg/mL (cP) | |
|---|---|---|---|---|
| Antibody 1 | 0.5 | −9.9 | <110 | 4.8 |
| Antibody 2 | 1 | 20.7 | ≥125 | 3.4 |
| Antibody 3 | 1 | 4.0 | ≥125 | 3.3 |
| Antibody 4 | 1.5 | 15.5 | ≥125 | 2.8 |
| Antibody 5 | 1.5 | 12.0 | ≥125 | 3 |
| Antibody 6 | 1 | −23.2 | <75 | n/a |
| Antibody 7 | 1 | 6.3 | ≥100b | 3.7 |
| Antibody 8 | 1 | 17.1 | ≥125 | Not testedb |
| IL330396c | 29.5 | – | – | – |
| NIP228d | 1.5 | – | – | – |
a. Solubility limitations prevented viscosity screening at desired concentration
b. Material availability limited extent of testing
c. Positive control antibody for the AC-SINS assay
d. Negative control antibody for the AC-SINS assay