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. 2021 Mar 17;1(3):e55. doi: 10.1002/cpz1.55

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© 2021 The Authors.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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MultiBac‐Based VLP‐factory™. On the right, the MultiBac baculoviral genome customized for enveloped virus‐like particle production is shown schematically. The capsid‐forming influenza H1N1 matrix protein M1 and the fluorescent protein mCherry are integrated into the viral backbone. The mCherry protein is a reporter for tracking virus performance. Influenza surface protein (hemagglutinin HA and/or neuraminidase NA) insertion into this VLP‐factory™ by means of transfer plasmids results in baculoviruses producing influenza virus‐like particles (VLPs). Electron micrographs of a selection of the VLPs is shown below. Recombinant influenza VLPs produced by the customized MultiBac genome (VLP‐factory™) and live influenza virus are close to identical in terms of size, shape, and appearance (inset, image courtesy of R. Ruigrok). Scale bars (50 μm) are drawn in white (adapted from Sari‐Ak et al., 2019).