Figure 3.

Overview of VLP‐factory™ protocol. VLP‐factory™ was created for the expression of a functional influenza VLP variant assay. A transfer plasmid library including genes encoding wild‐type and mutant influenza HA5 and HAB proteins was transformed into VLP‐factory™ (Basic Protocol 1). Illustrated is the initial virus in 6‐well plates, already giving the signal for the expression of mCherry that we perceive from the red color change (Basic Protocol 2). Parallel expression enables extraction and purification of the influenza VLPs by sedimentation and gradient centrifugation for functional studies (Basic Protocol 3).