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. 2021 Mar 17;1(3):e55. doi: 10.1002/cpz1.55

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© 2021 The Authors.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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VLP‐factory™‐produced influenza VLPs are functionally active. By Western blotting, the presence of HA protein in HA5 M1 VLP preparation was examined with a specific antibody (H5N1 MIA‐0052) showing the presence of specific HA bands (left). The right lane in the Western blot is the control containing VLPs comprising M1 only, showing no HA signal. M stands for molecular weight marker (sizes sin kDa). Recombinant influenza VLP was analyzed in a hemolysis experiment (middle). The y axis denotes the percentage of lysed red blood cells (RBC) in the experiment. The ELISA plot (right) shows antibody titers (IgG1) after two injections (adapted from Sari‐Ak et al., 2019). The y axis represents the level of antibody measured in these experiments.