Figure 6.

ADDomer^© VLP production. MultiBac‐based production of ADDomer^©. VLP production (Basic Protocol 4) is shown schematically, including a Coomassie‐stained SDS‐PAGE section of highly purified VLP. Plasmid pACEBac‐ADDomer comprises the synthetic gene encoding ADDomer^© in a BioBrick format as indicated (adapted from Vragniau et al., 2019). EMBacY is a version of the MultiBac baculovirus comprising a YFP reporter gene in the viral backbone. VL, insertion site engineered in variable loop; RGD1 and RDG2, insertion sites in RGD loop; Tn7L, Tn7R and mini‐attTn7, recognition and attachment sequences for Tn7 transposase; LoxP, site‐specific recombination site for Cre enzyme; Gent^R, Gentamicin resistance gene; Kan, Kanamycin; Amp, Ampicillin; LacZ, gene for blue/white screening of composite genome; ori^ColE1, replication origin; YFP, yellow fluorescent protein; M, molecular weight marker (sizes given in kDa).