Figure 8.

Native‐like peptide epitope presentation on ADDomer‐tevCHIK. Chikungunya envelope protein E2 consisting of three domains is shown (A, B, and C). E2EP3 (comprises the N‐terminus of E2 with the amino acid sequence NH2‐STDKDNFNVYKATRPYLAH). This E2E3P peptide, the major neutralizing epitope in the Chikungunya virus, is presented in an exposed native‐like conformation in ADDomer‐tevCHIK, with native‐like meaning that the N‐terminal serine residue of the epitope is accessible exactly as is the case in the Chikungunya viral envelope protein containing the major neutralizing epitope E2EP3. The N‐terminus was liberated by TEV protease cleavage at a specific site as indicated.