Table 1.

MultiBac Donor and Acceptor Plasmids

Vector names	Host strain	Replicon	Recombination and multiplication sides	Antibiotic resistance genes (1000× stock concentration)
Acceptor plasmids
pFL	TOP10 a	ColE1	Tn7L, Tn7R, loxP, multiplication module M	Ampicillin (100 mg/ml) Gentamycin (10 mg/ml)
pKL	TOP10	pBR322	Tn7L, Tn7R, loxP, multiplication module M	Kanamycin (50 mg/ml) Gentamycin (10 mg/ml)
Donor plasmids
pUCDM	BW23474 b	R6Kγ	loxP, multiplication module M	Chloramphenicol (30 mg/ml)
pSPL	BW23474	R6Kγ	loxP, multiplication module M	Spectinomycin (50 mg/ml)
Other plasmids
MultiBac bacmid	DH10MultiBac DH10MultiBacCre	F1	Tn7L, Tn7R, loxP	Kanamycin (50 mg/ml) Ampicillin (100 mg/ml)
pBADZ‐HisCre	TOP10 DH10MultiBacCre	ColE1	None	Zeomycin (50 mg/ml) (Zeocin)

^a recA^‐ endA^‐ pir^‐ other cloning strains can be used here as well.

^b recA^‐ endA^‐ pir⁺ other pir gene expressing strains can be used here as well.