Fig. 2.

Knockdown of KDM5A suppresses the malignancy of PRAD cells in vitro. A mRNA expression of KDM5A in PRAD cell lines (DU145, VCaP, PC-3 and C4-2) and in normal RWPE1 cells examined by RT-qPCR; B transfection efficacy of sh-KDM5A 1, 2, 3# in PRAD cells detected by RT-qPCR; C proliferation ability of DU145 and PC-3 cells detected by CCK-8 assay; D colony formation of DU145 and PC-3 cells examined by colony formation assay; E DNA replication ability of cells detected by EdU labeling assay; F apoptosis rate of DU145 and PC-3 cells determined by flow cytometry; G, migration ability of DU145 and PC-3cells measured by scratch test; H invasiveness of DU145 and PC-3 determined by Transwell assay; I angiogenesis ability of HUVECs in different CM detected by tube formation assay; J protein levels of apoptosis related factors (Bax and Bcl-2) and EMT-related factors (E-cadherin and Snail) in DU145 and PC-3 after sh-KDM5A transfection examined by western blot analysis. Data were collected from three independent experiments and expressed as mean ± SEM. Differences were analyzed by one-way ANOVA (A, B, C, D, E, F, G, H, I and J) or two-way ANOVA (C); #p < 0.05 compared to RWPE1; *p < 0.05 compared to sh-NC